FAP Protein (AA 26-760) (His-Avi Tag,Biotin)
Quick Overview for FAP Protein (AA 26-760) (His-Avi Tag,Biotin) (ABIN7274585)
Target
See all FAP ProteinsProtein Type
Origin
Source
Purity
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Protein Characteristics
- AA 26-760
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Purification tag / Conjugate
- This FAP protein is labelled with His-Avi Tag,Biotin.
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Purpose
- Biotinylated Human FAP Protein
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Sequence
- Leu26-Asp760
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Characteristics
- Recombinant Biotinylated Human FAP Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Leu26-Asp760.
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Sterility
- 0.22 μm filtered
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Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Biotinylated Human FAP, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FAP Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA. See testing image for detail.
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Fibroblast Activation Protein, alpha (FAP) (AA 26-760) protein (Fc Tag)
FAP Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Fibroblast Activation Protein, alpha (FAP) (AA 26-761) protein (His tag)FAP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Fibroblast Activation Protein, alpha (FAP) (AA 26-760) protein (His tag)FAP Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Activation Protein, alpha (FAP) (AA 26-760) protein (His-Avi Tag,Biotin)FAP Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Fibroblast Activation Protein, alpha (FAP) (AA 1-760) protein (His tag)custom-made FAP Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fibroblast Activation Protein, alpha (FAP) (AA 1-761) protein (His tag)custom-made FAP Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Fibroblast Activation Protein, alpha (FAP) (AA 30-760) protein (His tag)FAP Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Fibroblast Activation Protein, alpha (FAP) (AA 26-760) (Active) protein (Fc Tag)FAP Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fibroblast Activation Protein, alpha (FAP) (AA 26-760) (Active) protein (His tag)FAP Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Fibroblast Activation Protein, alpha (FAP) (AA 525-624) protein (GST tag)FAP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3 months after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Expiry Date
- 12 months
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- FAP (Fibroblast Activation Protein, alpha (FAP))
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Alternative Name
- FAP
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Background
- Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
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Molecular Weight
- 87.10 kDa. Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result.
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UniProt
- Q12884
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Pathways
- Tube Formation
Target
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