FcRn Protein (AA 24-297) (His tag)

Catalog No. ABIN7274675

Product Details

Target: FcRn (IgG receptor FcRn (FcRn))

Protein Type: Recombinant

Protein Characteristics: AA 24-297

Origin: Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purification tag / Conjugate: This FcRn protein is labelled with His tag.

Purpose: Cynomolgus/Rhesus macaque FcRn Protein

Sequence: Ala24-Ser297 (FCGRT) & Ile21-Met119 (B2M)

Specificity: Uni-Prot: Q8SPV9 (FCGRT), Q8SPW0 (B2M)

Characteristics: Recombinant Cynomolgus/Rhesus macaque FcRn Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala24-Ser297 (FCGRT) & Ile21-Met119 (B2M).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus/Rhesus macaque FcRn, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 13.3ng/ml determined by ELISA. The affinity constant of 0.18 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: FcRn (IgG receptor FcRn (FcRn))

Alternative Name: FcRn (FcRn Products)

Synonyms: FCRN Protein, alpha-chain Protein, FcRn Protein, Fc fragment of IgG receptor and transporter Protein, Fc receptor, IgG, alpha chain transporter Protein, FCGRT Protein, Fcgrt Protein

Background: The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.

Molecular Weight: 31.5 kDa (FCGRT)&11.6 kDa (B2M). Due to glycosylation, the protein migrates to 35-38 kDa (FCGRT)&13-15 kDa (B2M) based on Tris-Bis PAGE result.

UniProt: Q8SPV9

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process