FSTL3 Protein (AA 27-263) (His tag)
-
- Target See all FSTL3 Proteins
- FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-263
-
Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FSTL3 protein is labelled with His tag.
- Purpose
- Human FSTL3 Protein
- Sequence
- Met27-Val263
- Characteristics
- Recombinant Human FSTL3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met27-Val263.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human FSTL3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-FSTL3 Antibody, hFc Tag with the EC50 of 27.5ng/ml determined by ELISA. See testing image for detail.
-
Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3) (AA 24-256) protein (His tag)
FSTL3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3) (AA 27-263) protein (GST tag)FSTL3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3) (Active) protein (His tag)FSTL3 Origin: Mouse Host: HEK-293 Cells Recombinant > 85 % as determined by SDS-PAGE Active
Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3) (AA 27-263) protein (His tag)Crystallography grade FSTL3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3) (AA 24-256) protein (His tag)Crystallography grade FSTL3 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3) protein (Myc-DYKDDDDK Tag)FSTL3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
-
- Target
- FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))
- Alternative Name
- FSTL3 (FSTL3 Products)
- Synonyms
- E030038F23Rik Protein, Flrg Protein, FLRG Protein, FSRP Protein, follistatin-like 3 Protein, follistatin like 3 Protein, Fstl3 Protein, FSTL3 Protein
- Background
- Follistatin-like 3 (FSTL3) is a novel cytokine that regulates insulin sensitivity and counteracts activin/myostatin signalling. In the present study, regulation of FSTL3 in renal dysfunction was investigated in both human chronic kidney disease (CKD) and acute kidney dysfunction (AKD). Furthermore, mFSTL3 expression was analysed in insulin-sensitive tissues in a mouse model of CKD.
- Molecular Weight
- 26.1 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
-
