TNFRSF18 Protein (AA 26-161) (Biotin,His-Avi Tag)

Catalog No. ABIN7274788

This Recombinant TNFRSF18 protein is expressed in HEK-293 Cells.

Quick Overview for TNFRSF18 Protein (AA 26-161) (Biotin,His-Avi Tag) (ABIN7274788)

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 26-161

Purification tag / Conjugate: This TNFRSF18 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human GITR/TNFRSF18 Protein

Sequence: Gln26-Glu161

Characteristics: Recombinant Biotinylated Human GITR/TNFRSF18 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln26-Glu161.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human GITR Ligand Trimer, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GITR, His Tag with the EC50 of 77.9ng/ml determined by ELISA.,Biotinylated Human GITR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human GITR is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: TNFRSF18 (Tumor Necrosis Factor Receptor Superfamily, Member 18 (TNFRSF18))

Alternative Name: GITR

Background: GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a 40 kDa transmembrane glycoprotein that functions in immune regulation.GIRT is a receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.

Molecular Weight: 17.2 kDa. Due to glycosylation, the protein migrates to 26-28 kDa based on Bis-Tris PAGE result.

UniProt: Q9Y5U5

Pathways: Cancer Immune Checkpoints