HVEM Protein (AA 39-202) (His tag)
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- Target See all HVEM (TNFRSF14) Proteins
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 39-202
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This HVEM protein is labelled with His tag.
- Purpose
- Human HVEM/TNFRSF14 Protein
- Sequence
- Leu39-Val202
- Characteristics
- Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu39-Val202.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human HVEM, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Human BTLA, hFc Tag with the EC50 of 19.9ng/ml determined by ELISA. See testing image for detail.
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Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 39-202) protein (Fc Tag)
TNFRSF14 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 39-202) (Active) protein (His tag)TNFRSF14 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Func Active
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 38-200) protein (His-DYKDDDDK-Strep II Tag)TNFRSF14 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 39-202) (Active) protein (Fc Tag)TNFRSF14 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (Active) protein (Fc Tag)TNFRSF14 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) protein (His tag)TNFRSF14 Origin: Rhesus Monkey Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) protein (Fc Tag)TNFRSF14 Origin: Rhesus Monkey Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (AA 38-283) protein (GST tag)TNFRSF14 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14) (Active) protein (His tag,Fc Tag)TNFRSF14 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- HVEM (TNFRSF14) (Tumor Necrosis Factor Receptor Superfamily, Member 14 (TNFRSF14))
- Alternative Name
- HVEM (TNFRSF14 Products)
- Target Type
- Viral Protein
- Background
- Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
- Molecular Weight
- 18.5 kDa. Due to glycosylation, the protein migrates to 32-48 kDa based on Tris-Bis PAGE result.
- Pathways
- Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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