ICOS Protein (Cys136Ser-Mutant, Cys137Ser-Mutant) (His tag)

Catalog No. ABIN7274838

Product Details

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Protein Type: Recombinant

Protein Characteristics: Cys136Ser-Mutant, Cys137Ser-Mutant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ICOS protein is labelled with His tag.

Purpose: Human ICOS/CD278 (C136S, C137S) Protein

Sequence: Glu21-Phe141 (C136S,C137S)

Characteristics: Recombinant Human ICOS/CD278(C136S, C137S) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Phe141(C136S,C137S).

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human ICOS (C136S,C137S) , His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human B7-H2, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. The affinity constant of 40.2nM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS (ICOS Products)

Background: Inducible co-stimulator (ICOS), also called AILIM (activiation-inducible lymphocyte immunomediatory molecule) and CRP-1 (CD28-related protein-1), is a member of the growing CD28 family of immune costimulatory receptors. Other family members are CD28, CTLA-4 and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199 amino acids (aa) with a putative 20 aa signal sequence, a 121 aa extracellular domain, a 23 aa transmembrane region, and a 35 aa cytoplasmic domain.

Molecular Weight: 14.8 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.

Pathways: Cancer Immune Checkpoints