KLKB1 Protein (AA 20-638) (His tag)

Catalog No. ABIN7275094

Product Details

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Protein Type: Recombinant

Protein Characteristics: AA 20-638

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This KLKB1 protein is labelled with His tag.

Purpose: Cynomolgus KLKB1 Protein

Sequence: Gly20-Ala638

Characteristics: Recombinant Cynomolgus KLKB1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Ala638.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus KLKB1, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-KLKB1 Antibody, hFc Tag with the EC50 of 34.3g/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 20 mM NaAc, 150 mM NaCl ( pH 5.0).

Buffer: Lyophilized from 0.22μm filtered solution in 20 mM NaAc, 150 mM NaCl ( pH 5.0). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Alternative Name: KLKB1 (KLKB1 Products)

Background: Plasma kallikrein, also known as Fletcher factor or kallikrein B1 (KLKB1), is a serine endopeptidase, like its homologs tissue kallikrein and kallikrein-related peptidases (KLKs). Its physiological role is to catalyze the release of kinins and other vasoactive peptides.

Molecular Weight: 70.19 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5VTJ9

Pathways: Complement System, Brown Fat Cell Differentiation