LDLR Protein (AA 22-788) (His tag)
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- Target See all LDLR Proteins
- LDLR (Low Density Lipoprotein Receptor (LDLR))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 22-788
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Origin
- Cynomolgus
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This LDLR protein is labelled with His tag.
- Purpose
- Cynomolgus LDLR Protein
- Sequence
- Ala22-Gly788
- Characteristics
- Recombinant Cynomolgus LDLR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala22-Gly788.
- Purity
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- The affinity constant of 0.27 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- LDLR (Low Density Lipoprotein Receptor (LDLR))
- Alternative Name
- LDLR (LDLR Products)
- Background
- The low density lipoprotein receptor (LDLR) is the founding member of the LDL R family of widely expressed cell surface scavenger receptors. It is a cell-surface receptor that recognizes the apoprotein B100 which is embedded in the phospholipid outer layer of LDL particles.
- Molecular Weight
- 86 kDa, Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
- NCBI Accession
- XP_005588053
- Pathways
- Hepatitis C, Lipid Metabolism
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