MEPE Protein (AA 25-441) (His tag)
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- Target See all MEPE products
- MEPE (Matrix Extracellular phosphoglycoprotein (MEPE))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 25-441
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MEPE protein is labelled with His tag.
- Purpose
- Mouse MEPE Protein
- Sequence
- Ala25-Asp441
- Characteristics
- Recombinant Mouse MEPE Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Asp441.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
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Matrix Extracellular phosphoglycoprotein (MEPE) (AA 18-525) protein (His tag)
Crystallography grade MEPE Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Matrix Extracellular phosphoglycoprotein (MEPE) proteinMEPE Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
Matrix Extracellular phosphoglycoprotein (MEPE) proteinMEPE Origin: Rat Host: Escherichia coli (E. coli) Recombinant > 95 % SDS, WB, ELISA, IP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MEPE (Matrix Extracellular phosphoglycoprotein (MEPE))
- Alternative Name
- MEPE (MEPE Products)
- Synonyms
- OF45 Protein, OC-116 Protein, Of45 Protein, matrix extracellular phosphoglycoprotein Protein, matrix extracellular phosphoglycoprotein with ASARM motif (bone) Protein, MEPE Protein, Mepe Protein, LOC789774 Protein
- Background
- Matrix extracellular phosphoglycoprotein (MEPE) is expressed in bone and teeth where it has multiple functions. The C-terminus of MEPE contains a mineral-binding, acidic serine- and aspartate-rich motif (ASARM) that is also present in other noncollagenous proteins of mineralized tissues.MEPE-derived ASARM peptides function in phosphate homeostasis and direct inhibition of bone mineralization in a phosphorylation-dependent manner.
- Molecular Weight
- 45.3 kDa. The protein is cleaved into 2 chains and due to glycosylation, it migrates to 45-60 kDa based on Tris-Bis PAGE result.
- UniProt
- Q8K4L6
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