MUC16 Protein (AA 12660-12923) (His-Avi Tag,Biotin)
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- Target See all MUC16 (CA125) Proteins
- MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 12660-12923
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MUC16 protein is labelled with His-Avi Tag,Biotin.
- Purpose
- Biotinylated Human CA125/MUC16 Protein
- Sequence
- Gly12660-Met12923
- Characteristics
- Recombinant Biotinylated Human CA125/MUC16 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly12660-Met12923.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human MSLN, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 0.35μg/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product CA125 Protein
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Mucin 16, Cell Surface Associated (CA125) (AA 12660-12923) protein (His-Avi Tag)
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Mucin 16, Cell Surface Associated (CA125) (AA 13810-14451) protein (His tag)CA125 Origin: Human Host: Mammalian Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Mucin 16, Cell Surface Associated (CA125) (AA 1321-1415) protein (GST tag)CA125 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Mucin 16, Cell Surface Associated (CA125) protein (Biotin)CA125 Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Mucin 16, Cell Surface Associated (CA125) (AA 12660-12923) protein (Fc Tag,AVI tag,Biotin)CA125 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))
- Alternative Name
- CA125 (CA125 Products)
- Background
- MUC16, also known as the CA125 antigen, is a mucin protein that may be found in type I transmembrane or secreted forms that are used monitor the progress of epithelial ovarian cancer therapy.Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
- Molecular Weight
- 31.3 kDa. Due to glycosylation, the protein migrates to 52-80 kDa based on Tris-Bis PAGE result.
- UniProt
- Q8WXI7
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