Notch1 Protein (AA 19-526) (His-Avi Tag)

Catalog No. ABIN7275363

Product Details

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Protein Type: Recombinant

Protein Characteristics: AA 19-526

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This Notch1 protein is labelled with His-Avi Tag.

Purpose: Human Notch 1 Protein

Sequence: Ala19-Gln526

Characteristics: Recombinant Human Notch 1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala19-Gln526.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: The affinity constant of 0.48 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: Notch 1 (NOTCH1 Products)

Synonyms: notch1 Protein, NOTCH1 Protein, hn1 Protein, tan1 Protein, notch Protein, xotch Protein, xnotch Protein, notch-1 Protein, xnotch1 Protein, TAN1 Protein, hN1 Protein, 9930111A19Rik Protein, Mis6 Protein, N1 Protein, Tan1 Protein, lin-12 Protein, NOTCH Protein, Xotch Protein, notch1-a Protein, notch 1 Protein, fibropellin-1 Protein, Notch homolog 1, translocation-associated (Drosophila) Protein, Notch homolog 1, translocation-associated Protein, notch 1 S homeolog Protein, notch1 Protein, LOC100631526 Protein, NOTCH1 Protein, Notch1 Protein, notch1.S Protein

Background: Human Notch-1 is a 300 kDa type I transmembrane glycoprotein that is one of four human Notch homologues involved in developmental processes.Notch-1 functions as a receptor for membrane-bound ligands Jagged-1 (JAG1), Jagged-2 (JAG2) and Delta-1 (DLL1) to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Molecular Weight: 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development