ROR1 Protein (AA 30-305) (His tag)

Catalog No. ABIN7275529

Product Details

Target: ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))

Protein Type: Recombinant

Protein Characteristics: AA 30-305

Origin: Human, Rhesus Monkey, Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This ROR1 protein is labelled with His tag.

Purpose: Human/Cynomolgus/Rhesus macaque ROR1 (30-305,Ig-like & Frizzled Domain) Protein

Sequence: Gln30-Asp305

Characteristics: Recombinant Human/Cynomolgus/Rhesus macaque ROR1 (30-305,Ig-like & Frizzled Domain) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln30-Asp305.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human/Cynomolgus/Rhesus macaque ROR1 (30-305,Ig-like&Frizzled Domain) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-ROR1 Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: ROR1 (Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1))

Alternative Name: ROR1 (ROR1 Products)

Synonyms: NTRKR1 Protein, dJ537F10.1 Protein, 2810404D04Rik Protein, Ntrkr1 Protein, mRor1 Protein, RGD1559469 Protein, ROR1 Protein, fi34e02 Protein, si:dkey-209n16.1 Protein, wu:fi34e02 Protein, receptor tyrosine kinase like orphan receptor 1 Protein, receptor tyrosine kinase-like orphan receptor 1 Protein, ROR1 Protein, Ror1 Protein, ror1 Protein

Background: ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1),  is a member of  the ROR family within receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by the intracellular tyrosine kinase domains, highly related to those of the Trk-family receptor tyrosine kinases, and by the extracellular Frizzled-like cysteine-rich domains and kringle domains, which are common to receptors of the Wnt family members.

Molecular Weight: 31.90 kDa. Due to glycosylation, the protein migrates to 48-53 kDa based on Tris-Bis PAGE result.

Pathways: RTK Signaling, WNT Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha