P-Selectin Protein (His-Avi Tag,Biotin)
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- Target See all P-Selectin (SELP) Proteins
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This P-Selectin protein is labelled with His-Avi Tag,Biotin.
- Sequence
- Trp42-Ala771
- Purity
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Anti-P-selectin Antibody, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human P-Selectin, His Tag with the EC50 of 0.11μg/ml determined by ELISA. See testing image for detail.
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Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-709) protein (His tag)
SELP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (Fc Tag)SELP Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (Fc Tag)SELP Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 1-829) protein (GST tag)SELP Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (Active) protein (His tag)SELP Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) protein (His tag,AVI tag)SELP Origin: Mouse Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-709) protein (His tag)Crystallography grade SELP Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-771) protein (His tag)Crystallography grade SELP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-830) protein (rho-1D4 tag)Crystallography grade SELP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP) (AA 42-768) protein (rho-1D4 tag)Crystallography grade SELP Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % saccharose is added as protectant before lyophilization.
- Storage
- 4 °C,-80 °C
- Storage Comment
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))
- Alternative Name
- P-Selectin (SELP Products)
- Synonyms
- PSELECT Protein, CD62 Protein, CD62P Protein, GMP140 Protein, GRMP Protein, LECAM3 Protein, PADGEM Protein, PSEL Protein, GMP-140 Protein, Grmp Protein, selectin P Protein, selectin, platelet Protein, Selp Protein, SELP Protein
- Background
- FLJ45155, GMP140, GRMP, PADGEM, PSEL, P-Selectin, SELP, CD62P, CD62, LECAM3,Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
- Molecular Weight
- 82.9 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result.
- UniProt
- P16109
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