Sclerostin Protein (SOST) (AA 24-213) (His-Avi Tag)
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- Target See all Sclerostin (SOST) Proteins
- Sclerostin (SOST)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 24-213
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Sclerostin protein is labelled with His-Avi Tag.
- Purpose
- Human SOST/Sclerostin Protein
- Sequence
- Gln24-Tyr213
- Characteristics
- Recombinant Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus.It contains Gln24-Tyr213.
- Purity
- > 95 % as determined by Tris-Bis PAGE
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human SOST, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
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Sclerostin (SOST) (AA 24-213) protein (His-Avi Tag,Biotin)
SOST Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Sclerostin (SOST) (AA 69-258) protein (His tag)SOST Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Sclerostin (SOST) (AA 24-211) protein (His tag)SOST Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Sclerostin (SOST) protein (His tag)SOST Origin: Rat Host: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE
Sclerostin (SOST) (AA 24-213) protein (His tag,Biotin)SOST Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by reduced SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- Storage
- -20 °C,-80 °C
- Storage Comment
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Sclerostin (SOST)
- Alternative Name
- SOST (SOST Products)
- Background
- SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
- Molecular Weight
- 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.
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