alpha 2 Antiplasmin Protein (AA 27-490) (His tag)

Catalog No. ABIN7275642

Product Details

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Protein Type: Recombinant

Protein Characteristics: AA 27-490

Origin: Cynomolgus

Source: HEK-293 Cells

Purification tag / Conjugate: This alpha 2 Antiplasmin protein is labelled with His tag.

Purpose: Cynomolgus SERPINF2/A2AP Protein

Sequence: Met27-Lys490

Characteristics: Recombinant Cynomolgus SERPINF2/A2AP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met27-Lys490.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Cynomolgus SERPINF2, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SERPINF2 Antibody, hFc Tag with the EC50 of 0.29μg/ml determined by ELISA. See testing image for detail.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: alpha 2 Antiplasmin (SERPINF2) (Alpha2 Antiplasmin (SERPINF2))

Alternative Name: SERPINF2 (SERPINF2 Products)

Synonyms: A2AP Protein, AAP Protein, ALPHA-2-PI Protein, API Protein, PLI Protein, serpin Protein, AI747498 Protein, Pli Protein, Serpimf2 Protein, serpinf2 Protein, serpinf2l Protein, wu:fd57d08 Protein, Dmrs91 Protein, Pedf Protein, serpin family F member 2 Protein, serpin family F member 1 Protein, serine (or cysteine) peptidase inhibitor, clade F, member 2 Protein, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2a Protein, SERPINF2 Protein, Serpinf2 Protein, SERPINF1 Protein, serpinf2a Protein, Serpinf1 Protein

Background: Large-pool solvent/detergent (SD) plasma for transfusion exhibits reduced alpha 2-antiplasmin (alpha2-AP, SERPINF2) functional activity. The reason for the loss of alpha2-AP has not been described and could be due to the SD incubation itself and/or to the processing steps implemented to remove the solvent and the detergent.

Molecular Weight: 52.83 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

UniProt: A0A2K5WNU6

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones