Tissue factor Protein (AA 35-248) (His tag)

Catalog No. ABIN7275699

Product Details

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Protein Type: Recombinant

Protein Characteristics: AA 35-248

Origin: Dog

Source: HEK-293 Cells

Purification tag / Conjugate: This Tissue factor protein is labelled with His tag.

Purpose: Canine Coagulation Factor III/Tissue Factor Protein

Sequence: Thr35-Glu248

Characteristics: Recombinant Canine Coagulation Factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr35-Glu248.

Purity: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: Tissue factor (F3) (Coagulation Factor III (thromboplastin, Tissue Factor) (F3))

Alternative Name: Coagulation Factor III (F3 Products)

Synonyms: CD142 Protein, TF Protein, TFA Protein, PRO1557 Protein, PRO2086 Protein, TFQTL1 Protein, f3 Protein, AA409063 Protein, Cf-3 Protein, Cf3 Protein, tf Protein, zgc:112151 Protein, coagulation factor III, tissue factor Protein, transferrin Protein, coagulation factor IIIa Protein, coagulation factor III Protein, tissue factor Protein, coagulation factor IIIb Protein, coagulation factor III (thromboplastin, tissue factor) S homeolog Protein, F3 Protein, TF Protein, f3a Protein, tf Protein, f3b Protein, f3.S Protein

Background: Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.

Molecular Weight: 25.41 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.

UniProt: Q4W6L5

Pathways: Positive Regulation of Endopeptidase Activity, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling