SCG3 Protein (AA 20-468) (His tag)

Catalog No. ABIN7491167

Recombinant SCG3 protein expressed in HEK-293 Cells.

Quick Overview for SCG3 Protein (AA 20-468) (His tag) (ABIN7491167)

Target: SCG3 (Secretogranin III (SCG3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 20-468

Purification tag / Conjugate: This SCG3 protein is labelled with His tag.

Purpose: Recombinant human SCG3 protein with C-terminal 6xHis tag

Specificity: SCG3(Phe20-Leu468) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: SCG3 (Secretogranin III (SCG3))

Alternative Name: SCG3

Background: SGIII
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Granins may serve as precursors for biologically active peptides. Some granins have been shown to function as helper proteins in sorting and proteolytic processing of prohormones, however, the function of this protein is unknown. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Molecular Weight: predicted molecular mass of 51.8 kDa after removal of the signal peptide. The apparent molecular mass of SCG3-His is 55-70 kDa due to glycosylation.

UniProt: Q8WXD2