NAMPT Protein (AA 1-491) (His tag)

Catalog No. ABIN7504927

This Recombinant NAMPT protein is produced in Escherichia coli (E. coli).

Quick Overview for NAMPT Protein (AA 1-491) (His tag) (ABIN7504927)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-491

Purification tag / Conjugate: This NAMPT protein is labelled with His tag.

Sequence: Met1-His491

Characteristics: Recombinant Human PBEF is produced by our E.coli expression system and the target gene encoding Met1-His491 is expressed with a 6His tag at the N-terminus.

Endotoxin Level: <1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 um filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: PBEF

Background:

Abbreviation: PBEF,NAMPT

Target Synonym: Pre-B cell-enhancing factor,Nicotinamide phosphoribosyltransferase,NAmPRTase,Nampt,Pre-B-cell colony-enhancing factor 1,Visfatin,NAMPT,PBEF,PBEF1

Background: Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonal expansion and differentiation of pre-B cells, but it is also acknowledged to be the ubiquitous intracellular enzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine ""visfatin"". PBEF is constitutively expressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular and extracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induce angiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has been shown to increase inflammatory cytokines, such as TNF-α, IL-1β, IL-16, and TGF-β1. PBEF also increases the production of IL-6, TNF-α, and IL-1β in CD14+ monocyctes, macrophages, and dendritic cells, enhances the effectiveness of T cells.

Molecular Weight:

Calculated MW: 57 kDa

Observed MW: 55 kDa

UniProt: P43490