GAPDH Protein (AA 1-333) (His tag)
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- Target See all GAPDH Proteins
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-333
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This GAPDH protein is labelled with His tag.
- Application
- Immunogen (Imm)
- Sequence
- Met1-Glu333
- Characteristics
- A DNA sequence encoding the Human GAPDH (P04406) (1M-333E) was expressed with a polyhistidine tag at the N-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product GAPDH Protein
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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-333) protein (His tag)
GAPDH Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 2-335) protein (His tag)GAPDH Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) protein (GST tag)GAPDH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 226-335) protein (GST tag)GAPDH Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (AA 1-335) proteinGAPDH Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % pure SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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used as Immunogen for (ABIN6999908, ABIN7234256, ABIN7234257 and ABIN7234258)
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. - Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
- Expiry Date
- 12 months
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- Target
- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
- Alternative Name
- GAPDH (GAPDH Products)
- Background
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Abbreviation: GAPDH
Target Synonym: Aging associated gene 9 protein,Epididymis secretory sperm binding protein Li 162eP,G3P,G3PD,G3PDH,GAPD,GAPDH,Glyceraldehyde 3 phosphate dehydrogenase,Glyceraldehyde-3-phosphate dehydrogenase,HEL S 162eP,Peptidyl-cysteine S-nitrosylase GAPDH
Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
- Molecular Weight
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Calculated MW: 37.1 kDa
Observed MW: 34-37 kDa
- UniProt
- P04406
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