FAP Protein (AA 26-349) (His tag)

Catalog No. ABIN7505371

This Recombinant FAP protein is produced in Escherichia coli (E. coli).

Quick Overview for FAP Protein (AA 26-349) (His tag) (ABIN7505371)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Immunogen (Imm)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 26-349

Purification tag / Conjugate: This FAP protein is labelled with His tag.

Sequence: Leu26-Gly349

Characteristics: A DNA sequence encoding the Mouse FAP protein (P97321) (26L-349G) was expressed with a polyhistidine tag at the N-terminus.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAP

Background:

Abbreviation: FAP

Target Synonym: 170 kDa melanoma membrane bound gelatinase,170 kDa melanoma membrane-bound gelatinase,DPPIV,FAP,FAPA,Fibroblast activation protein alpha,Integral membrane serine protease,SEPR,Seprase,FAPalpha,SIMP

Background: Cell surface glycoprotein serine protease that participates in extracellular matrix degradation and involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth. Both plasma membrane and soluble forms exhibit post-proline cleaving endopeptidase activity, with a marked preference for Ala/Ser-Gly-Pro-Ser/Asn/Ala consensus sequences, on substrate such as alpha-2-antiplasmin SERPINF2 and SPRY2. Degrade also gelatin, heat-denatured type I collagen, but not native collagen type I and IV, vibronectin, tenascin, laminin, fibronectin, fibrin or casein. Have also dipeptidyl peptidase activity, exhibiting the ability to hydrolyze the prolyl bond two residues from the N-terminus of synthetic dipeptide substrates provided that the penultimate residue is proline, with a preference for Ala-Pro, Ile-Pro, Gly-Pro, Arg-Pro and Pro-Pro. Natural neuropeptide hormones for dipeptidyl peptidase are the neuropeptide Y (NPY), peptide YY (PYY), substance P (TAC1) and brain natriuretic peptide 32 (NPPB).

Molecular Weight:

Calculated MW: 38 kDa

Observed MW: 35-40 kDa

UniProt: P97321

Pathways: Tube Formation