PRKAA2 Protein (AA 16-195) (His tag)

Catalog No. ABIN7505470

Product Details

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Protein Type: Recombinant

Protein Characteristics: AA 16-195

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PRKAA2 protein is labelled with His tag.

Sequence: Tyr16-Val195

Characteristics: A DNA sequence encoding the Rat AMPK alpha2 (Q09137-1) (Tyr16-Val195) was expressed with a polyhistidine tag at the N-terminus.

Purity: > 85 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4., 5 % trehalose, 5 % mannitol, 0.01 % tween-80.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PRKAA2 (Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2))

Alternative Name: AMPK alpha2 (PRKAA2 Products)

Background:

Abbreviation: AMPK alpha2

Target Synonym: 5'-AMP-activated protein kinase catalytic subunit alpha-2,AAPK2,ACACA kinase,Acetyl-CoA carboxylase kinase,AMPK alpha 2 chain,AMPK subunit alpha-2,AMPK2,AMPKa2,AMPKalpha2,HMGCR kinase,Hydroxymethylglutaryl-CoA reductase kinase,PRKAA,PRKAA2,Protein kinase AMP activated alpha 2 catalytic subunit,Protein kinase AMP activated catalytic subunit alpha 2

Background: The protein encoded by this gene is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Molecular Weight:

Calculated MW: 20 kDa

Observed MW: 22.3 kDa

UniProt: Q09137-1

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, Warburg Effect