PCK1 Protein (AA 1-135) (His tag)

Catalog No. ABIN7505500

This Recombinant PCK1 protein is produced in Escherichia coli (E. coli).

Quick Overview for PCK1 Protein (AA 1-135) (His tag) (ABIN7505500)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Protein Type: Recombinant

Origin: Rat

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-135

Purification tag / Conjugate: This PCK1 protein is labelled with His tag.

Sequence: Met 1-Lys 135

Characteristics: A DNA sequence encoding the Rat PEPCK-C protein (P07379) (Met 1-Lys 135) was expressed with a N-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PEPCK-C

Background:

Abbreviation: PEPCK-C

Target Synonym: cytosolic [GTP],PCK1,PEP carboxykinase,PEPCK-C,PEPCKC,Phosphopyruvate carboxylase

Background: PCK1 (Phosphoenolpyruvate carboxykinase 1, also PEPCK-C [cytosolic]) is a monomeric, 67-68 kDa member of the PEP carboxykinase family of enzymes. It is expressed in postnatal cells such as mammary epithelium, white and brown adipocytes, skeletal muscle cells and hepatocytes. PCK1 has multiple functions, some of which are cell-specific. In particular, PCK1 has both cataplerotic (Greek: to fill down, or remove) and anaplerotic (to fill up, or replace) activity, where it removes and replaces elements of the TCA cycle. It is also gluconeogenic, and promotes glucose formation via PEP generation. Finally, it is glyceroneogenic, creating glycerol-3-phosphate that is used to reesterify and store just-released free fatty acids in adipocytes. It contains one kinase domain (aa 27-615), and two potential acetylation sites at Lys70 and 71. There are four potential splice forms. Two have alternative start sites at Met460 and Met315, while two others show a deletion of aa 34-546, plus a three aa substitution for aa 85-204, respectively.

Molecular Weight:

Calculated MW: 14.74 kDa

Observed MW: 15 kDa

UniProt: P07379

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis