GFRA1 Protein (Fc Tag,His tag)

Catalog No. ABIN7520038

This Recombinant GFRA1 protein is produced in HEK-293 Cells.

Quick Overview for GFRA1 Protein (Fc Tag,His tag) (ABIN7520038)

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 80 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This GFRA1 protein is labelled with Fc Tag,His tag.

Purpose: Active Recombinant Human GDNFR-alpha-1/GFRA1 Protein

Sequence: AEVSGGDRLD CVKASDQCLK EQSCSTKYRT LRQCVAGKET NFSLASGLEA KDECRSAMEA LKQKSLYNCR CKRGMKKEKN CLRIYWSMYQ SLQGNDLLED SPYEPVNSRL SDIFRVVPFI SVEHIPKGNN CLDAAKACNL DDICKKYRSA YITPCTTSVS NDVCNRRKCH KALRQFFDKV PAKHSYGMLF CSCRDIACTE RRRQTIVPVC SYEEREKPNC LNLQDSCKTN YICRSRLADF FTNCQPESRS VSSCLKENYA DCLLAYSGLI GTVMTPNYID SSSLSVAPWC DCSNSGNDLE ECLKFLNFFK DNTCLKNAIQ AFGNGSDVTV WQPAFPVQTT TATTTTALRV KNKPLGPAGS ENEIPTHVLP PCANLQAQKL KSNVSGNTHL CISNGNYEKE GLGASSHITT K

Specificity: Ala19-Lys429

Sterility: 0.22 μm filtered

Endotoxin Level: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human GDNF at 1 μg/mL (100 μL/well) can bind Human GFRA1 with a linear range of 2-332 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Alternative Name: GDNFR-alpha-1/GFRA1

Background:

Description: This protein is a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.

Name: GFRA1,GDNFR,GDNFRA,GFR-ALPHA-1,RET1L,RETL1,TRNR1

Gene ID: 2674

UniProt: P56159