METTL18 Protein (AA 1-372) (His tag)

Catalog No. ABIN7549366

This Recombinant METTL18 protein is produced in HEK-293 Cells.

Quick Overview for METTL18 Protein (AA 1-372) (His tag) (ABIN7549366)

Target: METTL18 (Methyltransferase Like 18 (METTL18))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-372

Purification tag / Conjugate: This METTL18 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant METTL18 Protein expressed in mammalian cells.

Sequence: MTFQFNFTIE DHLENELTPI RDGALTLDSS KELSVSESQK GEERDRKCSA EQFDLPQDHL WEHKSMENAA PSQDTDSPLS AASSSRNLEP HGKQPSLRAA KEHAMPKDLK KMLENKVIET LPGFQHVKLS VVKTILLKEN FPGENIVSKS FSSHSDLITG VYEGGLKIWE CTFDLLAYFT KAKVKFAGKK VLDLGCGSGL LGITAFKGGS KEIHFQDYNS MVIDEVTLPN VVANSTLEDE ENDVNEPDVK RCRKPKVTQL YKCRFFSGEW SEFCKLVLSS EKLFVKYDLI LTSETIYNPD YYSNLHQTFL RLLSKNGRVL LASKAHYFGV GGGVHLFQKF VEERDVFKTR ILKIIDEGLK RFIIEITFKF PG Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: METTL18 (Methyltransferase Like 18 (METTL18))

Alternative Name: METTL18

Background: Histidine protein methyltransferase 1 homolog (EC 2.1.1.85) (Arsenic-transactivated protein 2) (AsTP2) (Methyltransferase-like protein 18),FUNCTION: Protein-L-histidine N-tele-methyltransferase that specifically monomethylates RPL3, thereby regulating translation elongation (PubMed:23349634, PubMed:33693809, PubMed:35674491). Histidine methylation of RPL3 regulates translation elongation by slowing ribosome traversal on tyrosine codons: slower elongation provides enough time for proper folding of synthesized proteins and prevents cellular aggregation of tyrosine-rich proteins (PubMed:35674491). {ECO:0000269|PubMed:23349634, ECO:0000269|PubMed:33693809, ECO:0000269|PubMed:35674491}.

Molecular Weight: 42.1 kDa

UniProt: O95568

AlphaFold: O95568