RPH3A Protein (AA 1-694) (His tag)

Catalog No. ABIN7551541

This Recombinant RPH3A protein is produced in HEK-293 Cells.

Quick Overview for RPH3A Protein (AA 1-694) (His tag) (ABIN7551541)

Target: RPH3A (Rabphilin 3A (RPH3A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-694

Purification tag / Conjugate: This RPH3A protein is labelled with His tag.

Purpose: Predefined custom protein recombinant RPH3A Protein expressed in mammalian cells.

Sequence: MTDTVFSNSS NRWMYPSDRP LQSNDKEQLQ AGWSVHPGGQ PDRQRKQEEL TDEEKEIINR VIARAEKMEE MEQERIGRLV DRLENMRKNV AGDGVNRCIL CGEQLGMLGS ACVVCEDCKK NVCTKCGVET NNRLHSVWLC KICIEQREVW KRSGAWFFKG FPKQVLPQPM PIKKTKPQQP VSEPAAPEQP APEPKHPARA PARGDSEDRR GPGQKTGPDP ASAPGRGNYG PPVRRASEAR MSSSSRDSES WDHSGGAGDS SRSPAGLRRA NSVQASRPAP GSVQSPAPPQ PGQPGTPGGS RPGPGPAGRF PDQKPEVAPS DPGTTAPPRE ERTGGVGGYP AVGAREDRMS HPSGPYSQAS AAAPQPAAAR QPPPPEEEEE EANSYDSDEA TTLGALEFSL LYDQDNSSLQ CTIIKAKGLK PMDSNGLADP YVKLHLLPGA SKSNKLRTKT LRNTRNPIWN ETLVYHGITD EDMQRKTLRI SVCDEDKFGH NEFIGETRFS LKKLKPNQRK NFNICLERVI PMKRAGTTGS ARGMALYEEE QVERVGDIEE RGKILVSLMY STQQGGLIVG IIRCVHLAAM DANGYSDPFV KLWLKPDMGK KAKHKTQIKK KTLNPEFNEE FFYDIKHSDL AKKSLDISVW DYDIGKSNDY IGGCQLGISA KGERLKHWYE CLKNKDKKIE RWHQLQNENH VSSD Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RPH3A (Rabphilin 3A (RPH3A))

Alternative Name: RPH3A

Background: Rabphilin-3A (Exophilin-1),FUNCTION: Plays an essential role in docking and fusion steps of regulated exocytosis (By similarity). At the presynaptic level, RPH3A is recruited by RAB3A to the synaptic vesicle membrane in a GTP-dependent manner where it modulates synaptic vesicle trafficking and calcium-triggered neurotransmitter release (By similarity). In the post-synaptic compartment, forms a ternary complex with GRIN2A and DLG4 and regulates NMDA receptor stability. Also plays a role in the exocytosis of arginine vasopressin hormone (By similarity). {ECO:0000250|UniProtKB:P47709}.

Molecular Weight: 76.9 kDa

UniProt: Q9Y2J0

AlphaFold: Q9Y2J0

Pathways: Synaptic Vesicle Exocytosis