KLHL13 Protein (AA 1-655) (His tag)

Catalog No. ABIN7554365

This Recombinant KLHL13 protein is expressed in HEK-293 Cells.

Quick Overview for KLHL13 Protein (AA 1-655) (His tag) (ABIN7554365)

Target: KLHL13 (Kelch-Like 13 (KLHL13))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-655

Purification tag / Conjugate: This KLHL13 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant KLHL13 Protein expressed in mammalian cells.

Sequence: MPLKWKTSSP AIWKFPVPVL KTSRSTPLSP AYISLVEEED QHMKLSLGGS EMGLSSHLQS SKAGPTRIFT SNTHSSVVLQ GFDQLRLEGL LCDVTLMPGD TDDAFPVHRV MMASASDYFK AMFTGGMKEQ DLMCIKLHGV SKVGLRKIID FIYTAKLSLN MDNLQDTLEA ASFLQILPVL DFCKVFLISG VTLDNCVEVG RIANTYNLTE VDKYVNSFVL KNFPALLSTG EFLKLPFERL AFVLSSNSLK HCTELELFKA TCRWLRLEEP RMDFAAKLMK NIRFPLMTPQ ELINYVQTVD FMRTDNTCVN LLLEASNYQM MPYMQPVMQS DRTAIRSDTT HLVTLGGVLR QQLVVSKELR MYDEKAHEWK SLAPMDAPRY QHGIAVIGNF LYVVGGQSNY DTKGKTAVDT VFRFDPRYNK WMQVASLNEK RTFFHLSALK GYLYAVGGRN AAGELPTVEC YNPRTNEWTY VAKMSEPHYG HAGTVYGGVM YISGGITHDT FQKELMCFDP DTDKWIQKAP MTTVRGLHCM CTVGERLYVI GGNHFRGTSD YDDVLSCEYY SPILDQWTPI AAMLRGQSDV GVAVFENKIY VVGGYSWNNR CMVEIVQKYD PDKDEWHKVF DLPESLGGIR ACTLTVFPPE ETTPSPSRES PLSAP Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: KLHL13 (Kelch-Like 13 (KLHL13))

Alternative Name: KLHL13

Background: Kelch-like protein 13 (BTB and kelch domain-containing protein 2),FUNCTION: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis. {ECO:0000269|PubMed:14528312, ECO:0000269|PubMed:17543862, ECO:0000269|PubMed:19995937}.

Molecular Weight: 73.9 kDa

UniProt: Q9P2N7

AlphaFold: Q9P2N7