WTIP Protein (AA 1-430) (His tag)

Catalog No. ABIN7556032

This Recombinant WTIP protein is produced in HEK-293 Cells.

Quick Overview for WTIP Protein (AA 1-430) (His tag) (ABIN7556032)

Target: WTIP (Wilms Tumor 1 Interacting Protein (WTIP))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-430

Purification tag / Conjugate: This WTIP protein is labelled with His tag.

Purpose: Predefined custom protein recombinant WTIP Protein expressed in mammalian cells.

Sequence: MQRSRAGADE AALLLAGLAL RELEPGCGSP GRGRRGPRPG PGDEAAPALG RRGKGSGGPE AGADGLSRGE RGPRRAAVPE LSAQPAGSPR ASLAGSDGGG GGGSARSSGI SLGYDQRHGS PRSGRSDPRP GPGPPSVGSA RSSVSSLGSR GSAGAYADFL PPGACPAPAR SPEPAGPAPF PLPALPLPPG REGGPSAAER RLEALTRELE RALEARTARD YFGICIKCGL GIYGAQQACQ AMGSLYHTDC FTCDSCGRRL RGKAFYNVGE KVYCQEDFLY SGFQQTADKC SVCGHLIMEM ILQALGKSYH PGCFRCSVCN ECLDGVPFTV DVENNIYCVR DYHTVFAPKC ASCARPILPA QGCETTIRVV SMDRDYHVAC YHCEDCGLQL SGEEGRRCYP LAGHLLCRRC HLRRLQPGPL PSPTVHVTEL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: WTIP (Wilms Tumor 1 Interacting Protein (WTIP))

Alternative Name: WTIP

Background: Wilms tumor protein 1-interacting protein (WT1-interacting protein),FUNCTION: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates Hippo signaling pathway and antagonizes phosphorylation of YAP1. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. In podocytes, may play a role in the regulation of actin dynamics and/or foot process cytoarchitecture (By similarity). In the course of podocyte injury, shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype. Involved in the organization of the basal body (By similarity). Involved in cilia growth and positioning (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:A9LS46, ECO:0000269|PubMed:20303269, ECO:0000269|PubMed:20616046, ECO:0000269|PubMed:21834987, ECO:0000269|PubMed:22286099}.

Molecular Weight: 45.1 kDa

UniProt: A6NIX2

AlphaFold: A6NIX2