ZNF445 Protein (AA 1-1031) (His tag)

Catalog No. ABIN7556072

This Recombinant ZNF445 protein is expressed in HEK-293 Cells.

Quick Overview for ZNF445 Protein (AA 1-1031) (His tag) (ABIN7556072)

Target: ZNF445 (Zinc Finger Protein 445 (ZNF445))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1031

Purification tag / Conjugate: This ZNF445 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant ZNF445 Protein expressed in mammalian cells.

Sequence: MPPGRWHAAY PAQAQSSRER GRLQTVKKEE EDESYTPVQA ARPQTLNRPG QELFRQLFRQ LRYHESSGPL ETLSRLRELC RWWLRPDVLS KAQILELLVL EQFLSILPGE LRVWVQLHNP ESGEEAVALL EELQRDLDGT SWRDPGPAQS PDVHWMGTGA LRSAQIWSLA SPLRSSSALG DHLEPPYEIE ARDFLAGQSD TPAAQMPALF PREGCPGDQV TPTRSLTAQL QETMTFKDVE VTFSQDEWGW LDSAQRNLYR DVMLENYRNM ASLVGPFTKP ALISWLEARE PWGLNMQAAQ PKGNPVAAPT GDDLQSKTNK FILNQEPLEE AETLAVSSGC PATSVSEGIG LRESFQQKSR QKDQCENPIQ VRVKKEETNF SHRTGKDSEV SGSNSLDLKH VTYLRVSGRK ESLKHGCGKH FRMSSHHYDY KKYGKGLRHM IGGFSLHQRI HSGLKGNKKD VCGKDFSLSS HHQRGQSLHT VGVSFKCSDC GRTFSHSSHL AYHQRLHTQE KAFKCRVCGK AFRWSSNCAR HEKIHTGVKP YKCDLCEKAF RRLSAYRLHR ETHAKKKFLE LNQYRAALTY SSGFDHHLGD QSGEKLFDCS QCRKSFHCKS YVLEHQRIHT QEKPYKCTKC RKTFRWRSNF TRHMRLHEEE KFYKQDECRE GFRQSPDCSQ PQGAPAVEKT FLCQQCGKTF TRKKTLVDHQ RIHTGEKPYQ CSDCGKDFAY RSAFIVHKKK HAMKRKPEGG PSFSQDTVFQ VPQSSHSKEE PYKCSQCGKA FRNHSFLLIH QRVHTGEKPY KCRECGKAFR WSSNLYRHQR IHSLQKQYDC HESEKTPNVE PKILTGEKRF WCQECGKTFT RKRTLLDHKG IHSGEKRYKC NLCGKSYDRN YRLVNHQRIH STERPFKCQW CGKEFIGRHT LSSHQRKHTR AAQAERSPPA RSSSQDTKLR LQKLKPSEEM PLEDCKEACS QSSRLTGLQD ISIGKKCHKC SICGKTFNKS SQLISHKRFH TRERPFKCSK CGKTFRWSSN LARHMKNHIR D Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ZNF445 (Zinc Finger Protein 445 (ZNF445))

Alternative Name: ZNF445

Background: Zinc finger protein 445 (ZFP445) (Zinc finger protein 168) (Zinc finger protein with KRAB and SCAN domains 15),FUNCTION: Transcription regulator required to maintain maternal and paternal gene imprinting, a process by which gene expression is restricted in a parent of origin-specific manner by epigenetic modification of genomic DNA and chromatin, including DNA methylation. Acts by controlling DNA methylation during the earliest multicellular stages of development at multiple imprinting control regions (ICRs) (PubMed:30602440). Acts together with ZFP57, but seems to be the major factor in human early embryonic imprinting maintenance. In contrast, in mice, ZFP57 plays the predominant role in imprinting maintenance (PubMed:30602440). {ECO:0000269|PubMed:30602440}.

Molecular Weight: 119.0 kDa

UniProt: P59923

AlphaFold: P59923