ATOH7 Protein (AA 1-149) (His tag)

Catalog No. ABIN7556425

This Recombinant ATOH7 protein is produced in HEK-293 Cells.

Quick Overview for ATOH7 Protein (AA 1-149) (His tag) (ABIN7556425)

Target: ATOH7 (Atonal Homolog 7 (ATOH7))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-149

Purification tag / Conjugate: This ATOH7 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Atoh7 Protein expressed in mammalian cells.

Sequence: MKSACKPHGP PAGARGAPPC AGAAERAVSC AGPGRLESAA RRRLAANARE RRRMQGLNTA FDRLRRVVPQ WGQDKKLSKY ETLQMALSYI IALTRILAEA ERDWVGLRCE QRGRDHPYLP FPGARLQVDP EPYGQRLFGF QPEPFPMAS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ATOH7 (Atonal Homolog 7 (ATOH7))

Alternative Name: Atoh7

Background: Transcription factor Atoh7 (Atonal bHLH transcription factor 7) (Helix-loop-helix protein mATH-5) (mATH5) (Protein atonal homolog 7),FUNCTION: Transcription factor that binds to DNA at the consensus sequence 5'-CAG[GC]TG-3' (PubMed:33712461). Dimerization with TCF3 isoform E47 may be required in certain situations (By similarity). Binds to gene promoters and enhancer elements, and thereby regulates a transcriptional program of retinal ganglion cell (RGC) determinant genes (PubMed:33712461). Although the exact mechanism is not certain, retinal transcription regulation by ATOH7 has a role in RGC determination and survival, photoreceptor population development, targeting of RGC axons to the optic nerve and development of the retino-hypothalamic tract (PubMed:11493566, PubMed:11156601, PubMed:12451142, PubMed:33712461). Binds to its own promoter and enhancer sequences, suggesting autoregulation of ATOH7 transcription (PubMed:33712461). Required for retinal circadian rhythm photoentrainment (PubMed:12451142, PubMed:26392540). Plays a role in brainstem auditory signaling and binaural processing (PubMed:17977745). {ECO:0000250|UniProtKB:Q8N100, ECO:0000269|PubMed:11156601, ECO:0000269|PubMed:11493566, ECO:0000269|PubMed:12451142, ECO:0000269|PubMed:17977745, ECO:0000269|PubMed:26392540, ECO:0000269|PubMed:33712461}.

Molecular Weight: 16.6 kDa

UniProt: Q9Z2E5

AlphaFold: Q9Z2E5