SP8 Protein (AA 1-486) (His tag)

Catalog No. ABIN7558770

Recombinant SP8 protein expressed in HEK-293 Cells.

Quick Overview for SP8 Protein (AA 1-486) (His tag) (ABIN7558770)

Target: SP8 (Sp8 Transcription Factor (SP8))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-486

Purification tag / Conjugate: This SP8 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Sp8 Protein expressed in mammalian cells.

Sequence: MLAATCNKIG SPSPSPSSLS DSSSSFGKGF HPWKRSSSSS SGSCNVVGSS LSSFGVSGAS RNGGSSSAAA AAAAAAAAAA ALVSDSFSCG GSPGSSAFSL TSSSAAAAAA AAAAAASSSP FANDYSVFQA PGVSGGSGGG GGGGGGGSGA HSQDSSHQPV FISKVHTSVD GLQGIYPRVG MAHPYESWFK PSHPGLGAAA DVGSAGASSW WDVGAGWIDV QNPNGAAALP GSLHPAAGGL QTSLHSPLGG YNSDYSGLSH SAFSSGASSH LLSPAGQHLM DGFKPVLPGS YPDSAPSPLA GAGSSMLSAG PAAQLGGSPR SSARRYSGRA TCDCPNCQEA ERLGPAGASL RRKGLHSCHI PGCGKVYGKT SHLKAHLRWH TGERPFVCNW LFCGKRFTRS DELQRHLRTH TGEKRFACPV CNKRFMRSDH LSKHVKTHSG GGGSAGSGGG KKGSDTDSEH SAAGSPPCHS PELLQPPEPG HRNGLE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SP8 (Sp8 Transcription Factor (SP8))

Alternative Name: Sp8

Background: Transcription factor Sp8,FUNCTION: Transcription factor which plays a key role in limb development. Positively regulates FGF8 expression in the apical ectodermal ridge (AER) and contributes to limb outgrowth in embryos. {ECO:0000269|PubMed:15358670}.

Molecular Weight: 48.4 kDa

UniProt: Q8BMJ8

AlphaFold: Q8BMJ8