NEK11 Protein (AA 1-628) (His tag)

Catalog No. ABIN7559085

Recombinant NEK11 protein expressed in HEK-293 Cells.

Quick Overview for NEK11 Protein (AA 1-628) (His tag) (ABIN7559085)

Target: NEK11 (NIMA (Never In Mitosis Gene A)-Related Kinase 11 (NEK11))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-628

Purification tag / Conjugate: This NEK11 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Nek11 Protein expressed in mammalian cells.

Sequence: MLKFQETAKC VGRRPTVIPM YPTALIARRY VLQQKLGSGS FGTVYLVSDK KAKPGEELKV LKEISVGELN PNETVQANVE AQLLSRLHHP AIVRFHASFM EQETFCIITE YCEGRDLDYR IQEYKEAGKV FAENQIVEWF IQLLLGVDYM HERRILHRDL KSKNIFLKNN LLKIGDFGVS RLLMGSCELA TTLTGTPHYM SPEALKHQGY DAKSDIWSLA CILYEMCCLD HAFAGSSFLS VVLNIVEGKT PSLPDRYPRE LNTIMERMLN KSPSLRPSAA DILKAPYMEE QLQLLMCKYP EMTLEDKNSV CQKEAAHTIN AVQKKLHLQT LQALSDTQKT TPRERMWLRK LQAADERARR LKKIAEENYK ENDKRMQALR SRNVGSVHAH VLHELDERTL ESLPEPQSLP CLDLDELEPS LEDTIVDLGH YEIPEDPLVA EQYYSDVFDS CSEDSEEQEE EMIFSEAGGD TKEEESPSVY RTNQQDSDTA ALVGCLEHVL GYTSLDTKTI TNAVTDMSPG PMVFNSAVAR TKMKRMKESA VQKLGMETFE EVYDYLKRAR HQNAREAEIW EHLETVVPRA SDCFEVDQLL YFEELLLTME GKEPSLQNLP CEAAQKKPVK GTHFCDNP Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NEK11 (NIMA (Never In Mitosis Gene A)-Related Kinase 11 (NEK11))

Alternative Name: Nek11

Background: Serine/threonine-protein kinase Nek11 (EC 2.7.11.1) (Never in mitosis A-related kinase 11) (NimA-related protein kinase 11),FUNCTION: Protein kinase which plays an important role in the G2/M checkpoint response to DNA damage. Controls degradation of CDC25A by directly phosphorylating it on residues whose phosphorylation is required for BTRC-mediated polyubiquitination and degradation. {ECO:0000250|UniProtKB:Q8NG66}.

Molecular Weight: 71.6 kDa

UniProt: Q8C0Q4

AlphaFold: Q8C0Q4