ATG10 Protein (AA 1-215) (His tag)

Catalog No. ABIN7559677

This Recombinant ATG10 protein is produced in HEK-293 Cells.

Quick Overview for ATG10 Protein (AA 1-215) (His tag) (ABIN7559677)

Target: ATG10 (Autophagy Related 10 (ATG10))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-215

Purification tag / Conjugate: This ATG10 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Atg10 Protein expressed in mammalian cells.

Sequence: MEDEFFGEKS FQHYCAEFIR HSQQIGDGWE WRTAKECSDG YMCKTQFRIK NEASTPHVGT PASVLTCLPT EENLELPMDD SEVTRPAAVA EVIKHEYHVL YSCSYQVPVL YFRASFLDGR PLALEDIWEG VHECYKPRLL QGPWDTITQQ EHPILGQPFF VLHPCKTNEF MTAVLKNSQK INRNVNYITS WLSLVGPVVG LNLPLSYAKA TSQSE Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ATG10 (Autophagy Related 10 (ATG10))

Alternative Name: Atg10

Background: Ubiquitin-like-conjugating enzyme ATG10 (EC 2.3.2.-) (Autophagy-related protein 10) (APG10-like) (mAPG10),FUNCTION: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Plays a role in adenovirus-mediated cell lysis. {ECO:0000269|PubMed:12890687, ECO:0000269|PubMed:20723759, ECO:0000269|PubMed:21367888}.

Molecular Weight: 24.6 kDa

UniProt: Q8R1P4

AlphaFold: Q8R1P4

Pathways: ER-Nucleus Signaling, Autophagy