POLD4 Protein (AA 1-107) (His tag)

Catalog No. ABIN7560674

This Recombinant POLD4 protein is expressed in HEK-293 Cells.

Quick Overview for POLD4 Protein (AA 1-107) (His tag) (ABIN7560674)

Target: POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-107

Purification tag / Conjugate: This POLD4 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pold4 Protein expressed in mammalian cells.

Sequence: MGRKRFITDS YPVVKKREGP PGHSKGELAP ELGEDTQSLS QEETELELLR QFDLAWQYGP CTGITRLQRW SRAEQMGLKP PLEVYQVLKA HPEDPHFQCS LWHLYPL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit (POLD4))

Alternative Name: Pold4

Background: DNA polymerase delta subunit 4 (DNA polymerase delta subunit p12),FUNCTION: As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity. Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb. Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites. Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival. {ECO:0000250|UniProtKB:Q9HCU8}.

Molecular Weight: 12.4 kDa

UniProt: Q9CWP8

AlphaFold: Q9CWP8

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA