MED10 Protein (AA 1-135) (His tag)

Catalog No. ABIN7560728

This Recombinant MED10 protein is expressed in HEK-293 Cells.

Quick Overview for MED10 Protein (AA 1-135) (His tag) (ABIN7560728)

Target: MED10 (Mediator Complex Subunit 10 (MED10))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-135

Purification tag / Conjugate: This MED10 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Med10 Protein expressed in mammalian cells.

Sequence: MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLSQK LNFIVTGLQD IDKCRQQLHD ITVPLEVFEY IDQGRNPQLY TKECLERALA KNEQVKGKID TMKKFKSLLI QELSKVFPED MAKYRSIRGE DHPPS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MED10 (Mediator Complex Subunit 10 (MED10))

Alternative Name: Med10

Background: Mediator of RNA polymerase II transcription subunit 10 (Mediator complex subunit 10),FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity). {ECO:0000250}.

Molecular Weight: 15.7 kDa

UniProt: Q9CXU0

AlphaFold: Q9CXU0

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha