GINS1 Protein (AA 1-196) (His tag)

Catalog No. ABIN7560769

This Recombinant GINS1 protein is produced in HEK-293 Cells.

Quick Overview for GINS1 Protein (AA 1-196) (His tag) (ABIN7560769)

Target: GINS1 (GINS Complex Subunit 1 (Psf1 Homolog) (GINS1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-196

Purification tag / Conjugate: This GINS1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Gins1 Protein expressed in mammalian cells.

Sequence: MFCEKAMELV RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA KSAGRGDLIP TVKFRHCALL RNRRCTIAYL YDRLLRIRAL RWEYGSVLPN SLRFHMSAEE TEWFNHYKKS LATYMRSLGG DEGLDITQDV KPPKSLYIEV RCLKDYGEFE VDDGTSVLLK KNSQHFLPRW KCEQLIRQGV LEHVLS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GINS1 (GINS Complex Subunit 1 (Psf1 Homolog) (GINS1))

Alternative Name: Gins1

Background: DNA replication complex GINS protein PSF1 (GINS complex subunit 1),FUNCTION: Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. {ECO:0000250|UniProtKB:Q14691}.

Molecular Weight: 22.9 kDa

UniProt: Q9CZ15

AlphaFold: Q9CZ15

Pathways: DNA Replication, Synthesis of DNA