Dual Specificity Phosphatase 3 (DUSP3) (AA 1-185) protein (His tag)

Catalog No. ABIN7561101

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for Dual Specificity Phosphatase 3 (DUSP3) (AA 1-185) protein (His tag) (ABIN7561101)

Target: Dual Specificity Phosphatase 3 (DUSP3)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-185

Purification tag / Conjugate: His tag

Purpose: Predefined custom protein recombinant Dusp3 Protein expressed in mammalian cells.

Sequence: MSSSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVVPRVYVG NASVAQDITQ LQKLGITHVL NAAEGRSFMH VNTSASFYED SGITYLGIKA NDTQEFNLSA YFERATDFID QALAHKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSTVRQNREI GPNDGFLAQL CQLNDRLAKE GKVKL Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Dual Specificity Phosphatase 3 (DUSP3)

Alternative Name: Dusp3

Target Type: Viral Protein

Background: Dual specificity protein phosphatase 3 (EC 3.1.3.16) (EC 3.1.3.48) (T-DSP11) (Vaccinia H1-related phosphatase) (VHR),FUNCTION: Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2 (By similarity). {ECO:0000250, ECO:0000269|PubMed:16845380}.

Molecular Weight: 20.5 kDa

UniProt: Q9D7X3

AlphaFold: Q9D7X3

Pathways: Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades