ST6GALNAC6 Protein (AA 1-333) (His tag)

Catalog No. ABIN7561698

Product Details

Target: ST6GALNAC6 (ST6 (Alpha-N-Acetyl-Neuraminyl-2,3-beta-Galactosyl-1,3)-N-Acetylgalactosaminide alpha-2,6-Sialyltransferase 6 (ST6GALNAC6))

Protein Type: Recombinant

Protein Characteristics: AA 1-333

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ST6GALNAC6 protein is labelled with His tag.

Purpose: Custom-made recombinant St6galnac6 Protein expressed in mammalian cells.

Sequence: MACSRPPSQC DPTTLPPGPP AGRWPLPFSR RRREMSSNKE QRSAVFVILF ALITILILYS SNSANEVFHY GSLRGRTRRP VNLKKWSFSS AYFPILGNKT LPSRCNQCVI ITSSSHLLGT KLGPEIERAE CTIRMNDAPT SGYSADVGNK TTFRVVAHSS VFRVLRKPQE FVNRTPETVF IFWGPPNKMQ KPQGSLLRVI QRAGLMFPNM EAYAVSPARM QQFDDLFRGE TGKDREKSHS WLSTGWFTMV IAVELCDHVH VYGMVPPDYC SQRPRLQRMP YHYYEPKGPD ECVTYIQNEH SRKGNHHRFI TEKRVFSSWA QLYGITFSHP SWT Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ST6GALNAC6 (ST6 (Alpha-N-Acetyl-Neuraminyl-2,3-beta-Galactosyl-1,3)-N-Acetylgalactosaminide alpha-2,6-Sialyltransferase 6 (ST6GALNAC6))

Alternative Name: St6galnac6 (ST6GALNAC6 Products)

Background: Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (EC 2.4.99.-) (GalNAc alpha-2,6-sialyltransferase VI) (ST6GalNAc VI) (ST6GalNAcVI) (Sialyltransferase 7F) (SIAT7-F),FUNCTION: Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc onto glycolipids, forming an alpha-2,6-linkage. Produces branched type disialyl structures by transfer of a sialyl group onto the GalNAc or GlcNAc residue inside backbone core chains having a terminal sialic acid with an alpha-2,3-linkage on Gal. ST6GalNAcVI prefers glycolipids to glycoproteins, predominantly catalyzing the biosynthesis of ganglioside GD1alpha from GM1b. Also has activity toward GD1a and GT1b, and can generate DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside) (PubMed:10702226). Besides GMb1, MSGG and other glycolipids, it shows activity towards sialyl Lc4Cer generating disialyl Lc4Cer, which can lead to the synthesis of disialyl Lewis a (Le(a)), suggested to be a cancer-associated antigen (By similarity). {ECO:0000250|UniProtKB:Q969X2, ECO:0000269|PubMed:10702226}.

Molecular Weight: 38.2 kDa

UniProt: Q9JM95