ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1A (ABCB1A) (AA 1-1276) protein (His tag)

Catalog No. ABIN7562958

Recombinant protein expressed in HEK-293 Cells.

Quick Overview for ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1A (ABCB1A) (AA 1-1276) protein (His tag) (ABIN7562958)

Target: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1A (ABCB1A)

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1276

Purification tag / Conjugate: His tag

Purpose: Predefined custom protein recombinant Abcb1a Protein expressed in mammalian cells.

Sequence: MELEEDLKGR ADKNFSKMGK KSKKEKKEKK PAVSVLTMFR YAGWLDRLYM LVGTLAAIIH GVALPLMMLI FGDMTDSFAS VGNVSKNSTN MSEADKRAMF AKLEEEMTTY AYYYTGIGAG VLIVAYIQVS FWCLAAGRQI HKIRQKFFHA IMNQEIGWFD VHDVGELNTR LTDDVSKINE GIGDKIGMFF QAMATFFGGF IIGFTRGWKL TLVILAISPV LGLSAGIWAK ILSSFTDKEL HAYAKAGAVA EEVLAAIRTV IAFGGQKKEL ERYNNNLEEA KRLGIKKAIT ANISMGAAFL LIYASYALAF WYGTSLVISK EYSIGQVLTV FFSVLIGAFS VGQASPNIEA FANARGAAYE VFKIIDNKPS IDSFSKSGHK PDNIQGNLEF KNIHFSYPSR KEVQILKGLN LKVKSGQTVA LVGNSGCGKS TTVQLMQRLY DPLDGMVSID GQDIRTINVR YLREIIGVVS QEPVLFATTI AENIRYGRED VTMDEIEKAV KEANAYDFIM KLPHQFDTLV GERGAQLSGG QKQRIAIARA LVRNPKILLL DEATSALDTE SEAVVQAALD KAREGRTTIV IAHRLSTVRN ADVIAGFDGG VIVEQGNHDE LMREKGIYFK LVMTQTAGNE IELGNEACKS KDEIDNLDMS SKDSGSSLIR RRSTRKSICG PHDQDRKLST KEALDEDVPP ASFWRILKLN STEWPYFVVG IFCAIINGGL QPAFSVIFSK VVGVFTNGGP PETQRQNSNL FSLLFLILGI ISFITFFLQG FTFGKAGEIL TKRLRYMVFK SMLRQDVSWF DDPKNTTGAL TTRLANDAAQ VKGATGSRLA VIFQNIANLG TGIIISLIYG WQLTLLLLAI VPIIAIAGVV EMKMLSGQAL KDKKELEGSG KIATEAIENF RTVVSLTREQ KFETMYAQSL QIPYRNAMKK AHVFGITFSF TQAMMYFSYA ACFRFGAYLV TQQLMTFENV LLVFSAIVFG AMAVGQVSSF APDYAKATVS ASHIIRIIEK TPEIDSYSTQ GLKPNMLEGN VQFSGVVFNY PTRPSIPVLQ GLSLEVKKGQ TLALVGSSGC GKSTVVQLLE RFYDPMAGSV FLDGKEIKQL NVQWLRAQLG IVSQEPILFD CSIAENIAYG DNSRVVSYEE IVRAAKEANI HQFIDSLPDK YNTRVGDKGT QLSGGQKQRI AIARALVRQP HILLLDEATS ALDTESEKVV QEALDKAREG RTCIVIAHRL STIQNADLIV VIQNGKVKEH GTHQQLLAQK GIYFSMVSVQ AGAKRS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1A (ABCB1A)

Alternative Name: Abcb1a

Background: ATP-dependent translocase ABCB1 (ATP-binding cassette sub-family B member 1A) (MDR1A) (Multidrug resistance protein 1A) (EC 7.6.2.2) (Multidrug resistance protein 3) (P-glycoprotein 3) (Phospholipid transporter ABCB1) (EC 7.6.2.1),FUNCTION: Translocates drugs and phospholipids across the membrane. Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (By similarity). {ECO:0000250|UniProtKB:P08183, ECO:0000269|PubMed:8898203}.

Molecular Weight: 140.6 kDa

UniProt: P21447

AlphaFold: P21447