SPAG5 Protein (AA 1-1165) (His tag)

Catalog No. ABIN7564189

This Recombinant SPAG5 protein is produced in HEK-293 Cells.

Quick Overview for SPAG5 Protein (AA 1-1165) (His tag) (ABIN7564189)

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1165

Purification tag / Conjugate: This SPAG5 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Spag5 Protein expressed in mammalian cells.

Sequence: MWRVKTLNLG LSPSPQKGKP AMSTPLRELK LQPEALADSG KGPSMISALT PYLCRLELKE RCNNSSPVDF INTENNFLSE QFSHPSTHIE ACQRESDPTP ESNSLFHTLE EAIETVDDFV VDPRDDSIVE SMVLLPFSLG QQQDLMLQAH LDTTAERTKS SLNESLGLED LVGKEVAPCV EDSLTEIVAI RPEQPTFQDP PLGPSDTEDA PVDLVPSENV LNFSLARLSP SAVLAQDFSV DHVDPGEETV ENRVLQEMET SFPTFPEEAE LGDQAPAANA EAVSPLYLTS SLVEMGPREA PGPTVEDASR IPGLESETWM SPLAWLEKGV NTSVMLQNLR QSLSFSSVLQ DAAVGNTPLA TCSVGTSFTP PAPLEVGTKD STSETERLLL GCRPPDLATL SRHDLEENLL NSLVLLEVLS HQLQAWKSQL TVPHREARDS STQTDSSPCG VTKTPKHLQD SKEIRQALLQ ARNVMQSWGL VSGDLLSLLH LSLTHVQEGR VTVSQESQRS KTLVSSCSRV LKKLKAKLQS LKTECEEARH SKEMALKGKA AAEAVLEAFR AHASQRISQL EQGLTSMQEF RGLLQEAQTQ LIGLHTEQKE LAQQTVSLSS ALQQDWTSVQ LNYGIWAALL SWSRELTKKL TAKSRQALQE RDAAIEEKKQ VVKEVEQVSA HLEDCKGQIE QLKLENSRLT ADLSAQLQIL TSTESQLKEV RSQHSRCVQD LAVKDELLCQ LTQSNKEQAT QWQKEEMELK HIQAELLQQQ AVLAKEVQDL RETVEFIDEE SQVAHRELGQ IESQLKVTLE LLRERSLQCE TLRDTVDSLR AELASTEAKH EKQALEKTHQ HSQELRLLAE QLQSLTLFLQ AKLKENKAES EIILPSTGSA PAQEHPLSND SSISEQTPTA AVDEVPEPAP VPLLGSVKSA FTRVASMASF QPTETPDLEK SLAEMSTVLQ ELKSLCSLLQ ESKEEATGVL QREICELHSR LQAQEEEHQE ALKAKEADME KLNQALCLLR KNEKELLEVI QKQNEKILGQ IDKSGQLINL REEVTQLTQS LRRAETETKV LQEALEGQLD PSCQLMATNW IQEKVFLSQE VSKLRVMFLE MKTEKEQLMD KYLSHRHILE ENLRRSDTEL KKLDDTIQHV YETLLSIPET MKSCKELQGL LEFLS Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SPAG5 (Sperm Associated Antigen 5 (SPAG5))

Alternative Name: Spag5

Background: Sperm-associated antigen 5 (Mastrin) (Mitotic spindle-associated protein p126) (MAP126),FUNCTION: Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. Involved in centriole duplication. Required for CDK5RAP22, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (By similarity). {ECO:0000250|UniProtKB:Q96R06}.

Molecular Weight: 130.0 kDa

UniProt: Q7TME2

AlphaFold: Q7TME2

Pathways: M Phase