DAAM2 Protein (AA 1-1115) (His tag)

Catalog No. ABIN7564247

This Recombinant DAAM2 protein is expressed in HEK-293 Cells.

Quick Overview for DAAM2 Protein (AA 1-1115) (His tag) (ABIN7564247)

Target: DAAM2 (Dishevelled Associated Activator of Morphogenesis 2 (DAAM2))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1115

Purification tag / Conjugate: This DAAM2 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Daam2 Protein expressed in mammalian cells.

Sequence: MALRKRSPHG LGFLCCFGGS DLPEIDLRDS HPLQYLEFSG PIPNPEELNV RFAELVDELD LTDKNREAVF ALPPEKKWQI YCSKRKEQED PNKLATSWPE YYIDRINAMA AMQNLYETED EETDKRNQVV EDLKTALRTQ PMRFVTRFID LEGLTCLLNF LRGMDHTTCE SRIHTSLIGC IKALMNNSQG RAHVLAQPEA ISIIAQSLRT ENSKTKVAVL EILGAVCLVP GGHKKVLQAM LHYQAYAAER TRFQTLLNEL DRSLGRYRDE VNLKTAIMSF INAVLNAGAG EDNLEFRLHL RYEFLMLGIQ PVIDKLRQHE NAILDKHLDF FEMVRNEDDL ELARRFDMVH IDTKSASQMF ELIHKKLKHT EAYPCLLSVL HHCLQMPYKR NGGYFQQWQL LDRILQQIVL QDERGVDPDL APLENFNVKN IVNMLINENE VKQWRDQAEK FRKEHMELMS RLERKERECE TKTLEKEEMM RTLNKMKDKL ARESQELRQA RGQVAELVAR HNESSTGPVS SPPPPGGPLT LSSSRTTNDL PPPPPPLPFD SCPPPPAPPL PPGGPPIPPG APPCFSSGPP PSHDPFSSNE APLRKKRIPQ PSHPLKSFNW VKLNEERVSG TVWNEIDDSQ VFRILDLEDF EKMFSAYQRH QACMQEGPQR ERGNVRDGGA ASRPLPAVEA SAHRTEKASR SMVSATGAKK ELGSTEDIYI TSRKVKELSV IDGRRAQNCI ILLSKLKLSN DEIRQAILRM DEQEDLAKDM LEQLLKFIPE KSDIDLLEEH KHEIERMARA DRFLYEMSRI DHYQQRLQAL FFKKKFQERL AEAKPKVEAI LLASRELTLS QRLKQMLEVV LAIGNFMNKG QRGGAYGFRV ASLNKIADTK SSIDRNISLL HYLIMILEKH FPDILNMPSE LKHLSEAAKV NLAELEKEVS ILRRGLRAVE VELEYQRHQA RDPNDKFVPV MSDFITVSSF SFSELEDQLN EARDKFAKAL THFGEQESKM QPDEFFGIFD TFLQAFLEAR QDLEAMRRRK EEDERRARME FMLKEQREKE RWQRQRKVLA GGALEESGEF DDLVSALRSG EVFDKDLSKF KRNRKRPGSQ VPEVTRERAI NRLNY Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DAAM2 (Dishevelled Associated Activator of Morphogenesis 2 (DAAM2))

Alternative Name: Daam2

Background: Disheveled-associated activator of morphogenesis 2,FUNCTION: Key regulator of the Wnt signaling pathway, which is required for various processes during development, such as dorsal patterning, determination of left/right symmetry or myelination in the central nervous system (PubMed:22227309, PubMed:24091014, PubMed:25754822). Acts downstream of Wnt ligands and upstream of beta-catenin (CTNNB1) (PubMed:22227309, PubMed:25754822). Required for canonical Wnt signaling pathway during patterning in the dorsal spinal cord by promoting the aggregation of Disheveled (Dvl) complexes, thereby clustering and formation of Wnt receptor signalosomes and potentiating Wnt activity (PubMed:22227309). During dorsal patterning of the spinal cord, inhibits oligodendrocytes differentiation via interaction with PIP5K1A (PubMed:25754822). Also regulates non-canonical Wnt signaling pathway (PubMed:24091014). Acts downstream of PITX2 in the developing gut and is required for left/right asymmetry within dorsal mesentery: affects mesenchymal condensation by lengthening cadherin-based junctions through WNT5A and non-canonical Wnt signaling, inducing polarized condensation in the left dorsal mesentery necessary to initiate gut rotation (PubMed:24091014). Together with DAAM1, required for myocardial maturation and sarcomere assembly (PubMed:26526197). Is a regulator of actin nucleation and elongation, filopodia formation and podocyte migration (By similarity). {ECO:0000250|UniProtKB:Q86T65, ECO:0000269|PubMed:22227309, ECO:0000269|PubMed:24091014, ECO:0000269|PubMed:25754822, ECO:0000269|PubMed:26526197}.

Molecular Weight: 128.4 kDa

UniProt: Q80U19

AlphaFold: Q80U19