SPAG1 Protein (AA 1-901) (His tag)

Catalog No. ABIN7564309

This Recombinant SPAG1 protein is produced in HEK-293 Cells.

Quick Overview for SPAG1 Protein (AA 1-901) (His tag) (ABIN7564309)

Target: SPAG1 (Sperm Associated Antigen 1 (SPAG1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-901

Purification tag / Conjugate: This SPAG1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Spag1 Protein expressed in mammalian cells.

Sequence: MTAKAKDCPS LWGFGTTKTF KIPIEHLDFK YIENCSDVKH LEKILYVLRS GEEGYYPELT EFCEKCLTNL APKSRALRKD KPAETASSFS AEEWEKIDSD LKSWVSEIKR EENTCHFHDP ENHPGVEDPL PPVRGSTCCP HSGKETYSKS KTAKKRIPRD YAEWDKFDVE KECSKIDEDY KEKTVINNKA HLSKIETKIE TAGLTEKEKS FLANREKGKG NEAFYSGDYE EAVMYYTRSL SALPTAIAYN NRAQAEIKLQ RWSSALEDCE KALELDPGNV KALLRRATTY KHQNKLQEAV DDLRKVLQVE PDNDLAKKTL SEVERDLKNS EPVSELQTKG KRMVIEEVEN SGDEGGKGSA DEREDGGSDE AAMGNIQKKL MVRRSEGGRR SRRGRTPGPR AEQQGGLRET ATASTGDSHY PEEPRAADNP SGLKRRGNEL FRGGQFAEAA AQYSVAIAQL EPTGSANADE LSILYSNRAA CYLKEGNCRD CIQDCNRALE LHPFSVKPLL RRAMAYETLE QYRNAYVDYK TVLQIDCGIQ LASDSANRIA RILTELDGSK WRERLPPIPA VPTSEPLRVW LPAAETPDQD PCPNNCMPSI TDEKMFQALK EEGNQLVKDK NYKDAISKYN ECLKINSKAC AIYTNRALCY LKLGQFEEAK LDCEQALQID GENVKASHRL ALAQKGLENC RESGVDPSQV LLSPDSSEAA RHLDTKNDTA PPSKGRERRR IQVQEVDGSS DEEPERPAEA SATSAPARDG VEDGGSAEPA EKLDVSKPTN AYEFGQVLST ISARKDEEAC AHLLAITAPK DLPLLLSNKL EGDTFLLLIQ SLKSHLVAKD PSLVYEHLLY LSKAERFKTM LTLINKGQKE QMAQLFDGLS DTQSDGLTAE DVQALRRQYE L Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SPAG1 (Sperm Associated Antigen 1 (SPAG1))

Alternative Name: Spag1

Background: Sperm-associated antigen 1 (Infertility-related sperm protein Spag-1) (TPR-containing protein involved in spermatogenesis) (TPIS),FUNCTION: May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). May play a role in fertilization. Binds GTP and has GTPase activity (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:Q07617}.

Molecular Weight: 100.7 kDa

UniProt: Q80ZX8

AlphaFold: Q80ZX8