LARS Protein (AA 1-1178) (His tag)

Catalog No. ABIN7564399

This Recombinant LARS protein is produced in HEK-293 Cells.

Quick Overview for LARS Protein (AA 1-1178) (His tag) (ABIN7564399)

Target: LARS (Leucyl-tRNA Synthetase (LARS))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1178

Purification tag / Conjugate: This LARS protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Lars1 Protein expressed in mammalian cells.

Sequence: MAGRKGTAKV DFLKEIEKEA QQKWEAEKVF EVSASRLEKQ KQSSKGKYFV TFPYPYMNGR LHLGHTFSLS KCEFAVGYQR LKGKSCLFPF GLHCTGMPIK ACADKLKREI ELYGCPPDFP EEEEEEEESS AKPGDIVVRD KAKGKKSKAA AKAGSSKYQW DIMKSLGLSD DDIVKFSEAE HWLDYFPPLA VQDLKTIGLK VDWRRSFITT DVNPYYDSFV RWQFLTLRER NKIKFGKRYT IYSPKDGQPC MDHDRQTGEG VGPQEYTLVK LKVLEPYPSK LSGLKGKNIF LVAATLRPET MFGQTNCWVR PDMKYIGFET ANGDIFICTQ RAARNMSYQG FTKHNGVVPV VKELMGEEIL GASLSAPLTC YKVVYVLPML TIKEDKGTGV VTSVPSDSPD DLAALRDLKK KQALRTKFGI RDDMVLPFEP VPVLEIPGIG NLPAVTVCDE LKIQSQNDRE KLAEAKEKLY LRGFYDGVML VDGFKGQKIQ HVKKTIQKNM IDAGDALIYM EPEKQVMSRS ADECVVALCD QWYLDYGDEN WKKQTFQCLK NMETFCEESR KNFEASLDWL QEHACSRTYG LGTRLPWDEQ WLIESLSDST IYMAFYTVAH LLQGGDLNGQ AESPLGIRPQ QMTKDVWDYV FFKDAPFPKT QIPKEKLDQL KQEFEFWYPV DLRASGKDLI PNHLSYYIYN HVAMWPEQSD KWPVSVRANG HLLLNSEKMS KSTGNFLTLS QAVDKFSADG MRLALADAGD TVEDANFVEA MADAGILRLY TWVEWVKEML ASCSSLRSGP ADSFNDRVFA SEMNAGIIKT DQNYEKMMFK EALKTGFFEF QAAKDKYREL ATEGMHRELV FRFIEVQTIL LTPFCPHLCE HIWTLLGKPD SIMHASWPVA GPVDESLIRS SQYLMEVAHD LRLRLKNYMM PAKGKKTDKQ PAQRPSHCTI YVAKNYPVWQ HITLTTLRSH FEANNGKLPD NKVIASELGS LPELKKYMKK VMPFVAMIKE NMEKKGPRVL DLELEFDEQA VLMENIVYLT NSLELEHIEV KFASEAEDKV REECCPGKPL NVFRTEPGVP VSLVNPQPSS GHFSTKIDIR QGDSCESIIR RLMKTDRGIK DLSKVKLMRF DDPLLGPRRV PVLGREHSEK TLISENAVFH VDLVSKKVHL TENGLRTDIG DTMVYLVH Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: LARS (Leucyl-tRNA Synthetase (LARS))

Alternative Name: Lars1

Background: Leucine--tRNA ligase, cytoplasmic (EC 6.1.1.4) (Leucyl-tRNA synthetase) (LeuRS),FUNCTION: Aminoacyl-tRNA synthetase that catalyzes the specific attachment of leucine to its cognate tRNA (tRNA(Leu)). It performs tRNA aminoacylation in a two-step reaction: Leu is initially activated by ATP to form a leucyl-adenylate (Leu-AMP) intermediate, then the leucyl moiety is transferred to the acceptor 3' end of the tRNA to yield leucyl-tRNA. To improve the fidelity of catalytic reactions, it is also able to hydrolyze misactivated aminoacyl-adenylate intermediates (pre-transfer editing) and mischarged aminoacyl-tRNAs (post-transfer editing). {ECO:0000250|UniProtKB:Q9P2J5}.

Molecular Weight: 134.2 kDa

UniProt: Q8BMJ2

AlphaFold: Q8BMJ2

Pathways: EGFR Signaling Pathway