ANAPC5 Protein (AA 1-740) (His tag)

Catalog No. ABIN7564427

Product Details

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Protein Type: Recombinant

Protein Characteristics: AA 1-740

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This ANAPC5 protein is labelled with His tag.

Purpose: Custom-made recombinant Anapc5 Protein expressed in mammalian cells.

Sequence: MMTNGVVHAN LFGIKDWVTP YKIAVLVLLN EMGRTGEGAV SLVERRKLNQ LLLPLLQGPD ITLSKLYKLI EESCPQLANS VQIRIKLMAE GELKDMEQFF DDLSDSFSGT EPEVHKTSVV GLFLRHMILA YSKLSFSQVF KLYTALQQYF QNGEKKTVED ADMDREDGEK QMEKEELDVS VREEEVSCSG PLSQKQAEFF LSQQAALLKN DETKALTPAS LQKELNNLLK FNPDFAEAHY LSYLNNLRVQ DVFSSTHSLL HYFDRLILTG AEGKSNGEEG YGRSLRYAAL NLAALHCRFG HYQQAELALQ EAIRIAQESN DHVCLQHCLS WLYVLGQKRA DSYVLLEHSV KKAVHFGLPY LASLGIQSLV QQRAFAGKTA NKLMDALKDS DLLHWKHSLS ELIDISIAQK TAIWRLYGRS TMALQQAQML LSMNSLESLN AGVQQNNTES FAVALCHLAE LHAEQGCFAA AGEVLKHLKD RFPPNSQHAQ LWMLCDQKIQ FDRAMNDGKF HLADSLVTGI TALNGIEGVY RKAVVLQAQN QMTEAHKLLQ KLLTYCQKLK NTEMVISVLL SVAELYWRSS SPTIAMPVLL EALALSKEYR LQYLASETVL NLAYAQLILG IPEQALTLLH MAIEPILADG AVLDKGRAMF LVSKCQVASA ASYDPVKKAE ALEAAIQNLS EAKNYFAQVD CRERIRDVAY FQARLYHALG KTQERNHCAM IFRQLHQELP AHGVPLINHL Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ANAPC5 (Anaphase Promoting Complex Subunit 5 (ANAPC5))

Alternative Name: Anapc5 (ANAPC5 Products)

Background: Anaphase-promoting complex subunit 5 (APC5) (Cyclosome subunit 5),FUNCTION: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity). {ECO:0000250}.

Molecular Weight: 83.1 kDa

UniProt: Q8BTZ4

AlphaFold: Q8BTZ4

Pathways: M Phase