CCDC99 Protein (AA 1-608) (His tag)

Catalog No. ABIN7564855

This Recombinant CCDC99 protein is produced in HEK-293 Cells.

Quick Overview for CCDC99 Protein (AA 1-608) (His tag) (ABIN7564855)

Target: CCDC99 (Coiled-Coil Domain Containing 99 (CCDC99))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-608

Purification tag / Conjugate: This CCDC99 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Spdl1 Protein expressed in mammalian cells.

Sequence: MEADITNLRN KLKECEDERL KAAHYGLQLL ERQTELQSQL DKCHEEMMIT AEKYNQEKHA LQREVELKSR MLDSLSCECE ALKQQQKAQL EQLEVQLHRS HRQEVSDLKN KLENLKVELD EARLGEKQLK QKLDLQGELL AHKSEELRLL SEQRVLSSMS SELLALETEL TAAEGVKNAL KEEVNELQYK QEQLECLNTS LLHQVDRLKE EKEEREREAV SYYNALEKAR VENQDLQVQL GHALQQAADP NSKGNSLFAE VEDRRVAMER QLNLMKDKYQ SLKKQNAFTR DQMNKMKLQI STLLRMRGSQ TEFEQQERLF AMIEQKNGEI KHLLGEINKL EKFKNLYESM ESRPSTSDTA CVLEDSTYYS DLLQLKLDKL NKENESTKDE LSIQRMKALF ESQRALDIER KLFTNERHLQ LSESENMKLR AKLDELKLKY EPEERIEVPV LKRRREVLPL NITTPEETEE TAAASATEDG VSRLPPHREE ESCLNSLKDN TVQWKQPASS CVQPASLSPH KNLHLDTQPK KEKKCVKLVD SPANIEVLHE QSGNTPNSPR LTAESKLPTE VKERIETTSK LGKGACKKSH NIIYVSSKSA PETQCSQQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: CCDC99 (Coiled-Coil Domain Containing 99 (CCDC99))

Alternative Name: Spdl1

Background: Protein Spindly (Coiled-coil domain-containing protein 99) (Spindle apparatus coiled-coil domain-containing protein 1),FUNCTION: Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (By similarity). Plays a role in cell migration (By similarity). {ECO:0000250|UniProtKB:Q96EA4, ECO:0000255|HAMAP-Rule:MF_03041}.

Molecular Weight: 70.2 kDa

UniProt: Q923A2

AlphaFold: Q923A2