TRIM33 Protein (AA 1-1142) (His tag)

Catalog No. ABIN7564925

This Recombinant TRIM33 protein is expressed in HEK-293 Cells.

Quick Overview for TRIM33 Protein (AA 1-1142) (His tag) (ABIN7564925)

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1142

Purification tag / Conjugate: This TRIM33 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Trim33 Protein expressed in mammalian cells.

Sequence: MAENKGGGEA ESGGGGSGSA PVTAGAAGPT AQEAEPPLAA VLVEEEEEEG GRAGAEGGAA GPDDGGVAAA SSSSAPAASV PAASVGSAVP GGAASTPAPA AAPAPAPAPA PAPAPAPAPA PAPGSSSGPP LGPPASLLDT CAVCQQSLQS RREAEPKLLP CLHSFCLRCL PEPERQLSVP IPGGSNGDVQ QVGVIRCPVC RQECRQIDLV DNYFVKDTSE APSSSDEKSE QVCTSCEDNA SAVGFCVECG EWLCKTCIEA HQRVKFTKDH LIRKKEDVSE SVGTSGQRPV FCPVHKQEQL KLFCETCDRL TCRDCQLLEH KEHRYQFLEE AFQNQKGAIE NLLAKLLEKK NYVHFAATQV QNRIKEVNET NKRVEQEIKV AIFTLINEIN KKGKSLLQQL ENVTKERQMK LLQQQNDITG LSRQVKHVMN FTNWAIASGS STALLYSKRL ITFQLRHILK ARCDPVPAAN GAIRFHCDPT FWAKNVVNLG NLVIESKPAP GYTPNVVVGQ VPPGTNHISK TPGQINLAQL RLQHMQQQVY AQKHQQLQQM RLQQPPAPIP TTTATTQQHP RQAAPQMLQQ QPPRLISVQT MQRGNMNCGA FQAHQMRLAQ NAARIPGIPR HSAPQYSMMQ PHLQRQHSNP GHAGPFPVVS AHNPINPTSP TTATMANANR GPTSPSVTAI ELIPSVTNPE NLPSLPDIPP IQLEDAGSSS LDNLLSRYIS GSHLPPQPTS TMNPSPGPSA LSPGSSGLSN SHTPVRPPST SSTGSRGSCG SSGRTAEKSA HSFKSDQVKV KQEPGTEEEI CSFSGAVKQE KTEDGRRSAC MLSSPESSLT PPLSTNLHLE SELDTLTGLE NHVKTEPTDI SESCKQSGLS NLVNGKSPIR NLMHRSARIG GDGNSKDDDP NEDWCAVCQN GGDLLCCEKC PKVFHLTCHV PTLLSFPSGD WICTFCRDIG KPEVEYDCDN MQHSKKGKTA QGLSPVDQRK CERLLLYLYC HELSIEFQEP VPVSIPNYYK IIKKPMDLST VKKKLQKKHS QHYQIPDDFV ADVRLIFKNC ERFNEMMKVV QVYADTQEIN LKGDSEVAKA GKAVALYFED KLSEIYSDRT FTPLPEFEQD EDDGEVTEDS DEDFIQPRRK RLKSDERPVH IK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TRIM33 (Tripartite Motif Containing 33 (TRIM33))

Alternative Name: Trim33

Background: E3 ubiquitin-protein ligase TRIM33 (EC 2.3.2.27) (Ectodermin homolog) (RING-type E3 ubiquitin transferase TRIM33) (Transcription intermediary factor 1-gamma) (TIF1-gamma) (Tripartite motif-containing protein 33),FUNCTION: Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity). May act as a transcriptional repressor (By similarity). Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity). Plays a role in the control of cell proliferation (By similarity). Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity). {ECO:0000250, ECO:0000269|PubMed:16751102}.

Molecular Weight: 123.8 kDa

UniProt: Q99PP7

AlphaFold: Q99PP7