MRGPRX2 Protein (DYKDDDDK Tag,Strep Tag)

Catalog No. ABIN7596584

This Synthetic Nanodisc MRGPRX2 protein is expressed in HEK-293 Cells.

Quick Overview for MRGPRX2 Protein (DYKDDDDK Tag,Strep Tag) (ABIN7596584)

Target: MRGPRX2 (G Protein-Coupled Receptor MRGX2 (MRGPRX2))

Protein Type: Synthetic Nanodisc

Origin: Human

Source: HEK-293 Cells

Application: ELISA, Cryogenic electron microscopy (cryo-EM), Immunogen (Imm), Phage Display (PhD), Surface Plasmon Resonance (SPR)

Product Details

Purification tag / Conjugate: This MRGPRX2 protein is labelled with DYKDDDDK Tag,Strep Tag.

Purpose: Human MRGPRX2-Strep full length protein-synthetic nanodisc

Application Details

Comment:

Advantages:

• Highly purified membrane proteins
• High solubility in aqueous solutions
• High stability
• Proteins are in a native membrane environment and remain biologically active
• No detergent and can be used for cell-based assays
• No MSP backbone proteins
• Mammalian cell expression system ensures post- translational modifications

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: MRGPRX2 (G Protein-Coupled Receptor MRGX2 (MRGPRX2))

Alternative Name: MRGPRX2

Background: MGRG3, MRGX2
Mast cell-specific receptor for basic secretagogues, i.e. cationic amphiphilic drugs, as well as endo- or exogenous peptides, consisting of a basic head group and a hydrophobic core. Recognizes and binds small molecules containing a cyclized tetrahydroisoquinoline (THIQ), such as non-steroidal neuromuscular blocking drugs (NMBDs), including tubocurarine and atracurium. In response to these compounds, mediates pseudo-allergic reactions characterized by histamine release, inflammation and airway contraction. Acts as a receptor for a number of other ligands, including peptides and alkaloids, such as cortistatin-14, proadrenomedullin N-terminal peptides PAMP-12 and, at lower extent, PAMP-20, antibacterial protein LL-37, PMX-53 peptide, beta-defensins, and complanadine A.

Molecular Weight: The human full length MRGPRX2-Strep protein has a MW of 37.1 kDa

UniProt: Q96LB1