Angiotensin I Converting Enzyme 1 Protein (AA 30-1232) (His tag)

Catalog No. ABIN7662733

This Recombinant Angiotensin I Converting Enzyme 1 protein is expressed in HEK-293 Cells.

Quick Overview for Angiotensin I Converting Enzyme 1 Protein (AA 30-1232) (His tag) (ABIN7662733)

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Western Blotting (WB), ELISA

Purity: Greater than 90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 30-1232

Purification tag / Conjugate: This Angiotensin I Converting Enzyme 1 protein is labelled with His tag.

Purpose: Recombinant Human ACE

Biological Activity Comment: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH. The specific activity is >8,00 pmol/min/μg.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5 % trehalose, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))

Alternative Name: ACE

Background: ACE, ACE1, Angiotensin converting enzyme, CD143, DCP, DCP1, Dipeptidyl carboxypeptidase I, Kininase II, MVCD3

Molecular Weight: 160-180 kDa

UniProt: P12821

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration