ABCB1 Protein (His tag)
Quick Overview for ABCB1 Protein (His tag) (ABIN7668202)
Target
See all ABCB1 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Purification tag / Conjugate
- This ABCB1 protein is labelled with His tag.
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Purpose
- Recombinant Human P-glycoprotein1/ABCB1 protein (His tag)
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Sequence
- Leu 392-Met 628
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Purification
- > 95 % as determined by reducing SDS-PAGE.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- < 10 EU/mg of the protein as determined by the LAL method.
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ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) (AA 1-1280) protein (His tag)
custom-made ABCB1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) (AA 620-709) protein (GST tag)ABCB1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) (AA 491-694) protein (His-IF2DI Tag)ABCB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) protein (DYKDDDDK Tag)ABCB1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) protein (His tag)ABCB1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Expiry Date
- 12 months
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- ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))
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Alternative Name
- P-glycoprotein1,ABCB1
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Background
- Translocates drugs and phospholipids across the membrane (PubMed:8898203, PubMed:2897240, PubMed:9038218, PubMed:35970996). Catalyzes the flop of phospholipids from the cytoplasmic to the exoplasmic leaflet of the apical membrane. Participates mainly to the flop of phosphatidylcholine, phosphatidylethanolamine, beta-D-glucosylceramides and sphingomyelins (PubMed:8898203). Energy-dependent efflux pump responsible for decreased drug accumulation in multidrug-resistant cells (PubMed:2897240, PubMed:9038218, PubMed:35970996).,ABCB1,ATP-binding cassette sub-family B member 1,ATP-dependent translocase ABCB1,CD243,P-glycoprotein 1,Phospholipid transporter ABCB1
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Molecular Weight
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calculated_mw: 26.0 kDa
observed_mw: 32 kDa
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UniProt
- P08183
Target
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