RAD51 Protein (His tag)

Catalog No. ABIN7668659

This Recombinant RAD51 protein is expressed in Escherichia coli (E. coli).

Quick Overview for RAD51 Protein (His tag) (ABIN7668659)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This RAD51 protein is labelled with His tag.

Purpose: Recombinant Human Rad51 protein (His tag)

Sequence: Ala 2-Asp 339

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Alternative Name: Rad51

Background: Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.,HsRAD51,DNA repair protein RAD51 homolog 1,RAD51,hRAD51

Molecular Weight:

calculated_mw: 37.1 kDa

observed_mw: 38 kDa

UniProt: Q06609

Pathways: DNA Damage Repair