WNT9B Protein (AA 23-357)

Catalog No. ABIN7866101

Recombinant WNT9B protein expressed in CHO Cells.

Quick Overview for WNT9B Protein (AA 23-357) (ABIN7866101)

Target: WNT9B (Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: CHO Cells

Purity: >95 % , as determined by SDS-PAGE and HPLC

Product Details

Protein Characteristics: AA 23-357

Purpose: Human WNT9B Protein Recombinant

Sequence: SYFGLTGREV LTPFPGLGTA AAPAQGGAHL KQCDLLKLSR RQKQLCRREP GLAETLRDAA HLGLLECQFQ FRHERWNCSL EGRMGLLKRG FKETAFLYAV SSAALTHTLA RACSAGRMER CTCDDSPGLE SRQAWQWGVC GDNLKYSTKF LSNFLGSKRG NKDLRARADA HNTHVGIKAV KSGLRTTCKC HGVSGSCAVR TCWKQLSPFR ETGQVLKLRY DSAVKVSSAT NEALGRLELW APARQGSLTK GLAPRSGDLV YMEDSPSFCR PSKYSPGTAG RVCSREASCS SLCCGRGYDT QSRLVAFSCH CQVQWCCYVE CQQCVQEELV YTCKH

Characteristics:

Protein Size: Recombinant WNT9B migrates as an approximately 37 kDa protein due to glycosylation under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human WNT9B mature chain including a C-terminal 6xHis tag was expressed in CHO. Recombinant Human Protein Wnt-9b encodes amino acid residues 23-357.

Endotoxin Level: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by its ability to induce alkaline phosphatase production by CCL-226 cells.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: WNT9B was Lyophilized from PBS, pH 7.2.

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Expiry Date: 24 months

Target Details

Target: WNT9B (Wingless-Type MMTV Integration Site Family, Member 9B (WNT9B))

Alternative Name: WNT9B

Molecular Weight: 37 kDa

Pathways: WNT Signaling, Tube Formation